Review

usuv eu3 (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC usuv eu3

a Weight loss is expressed in % of initial weight. The humane endpoint was set at 75% of initial weight (dashed line). Results averaged from three individuals infected with <t>USUV</t> Afr3 and <t>Eu3</t> and two control individuals, bars represent SD. Statistically significant difference was observed between the Eu3 and ctrls group on 6 d.p.i. as indicated by the asterisk * p = 0.03. b Survival curves of blackbirds. No statistically significant difference was observed in survival among the three groups of blackbirds.

Usuv Eu3, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 892 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/usuv+eu3/M%2E+dunni/pmc12181337-172-14-42
Average 96 stars, based on 892 article reviews

usuv eu3 - by Bioz Stars, 2026-09

96/100 stars

Images

1) Product Images from "Experimental Usutu virus infection in Eurasian blackbirds ( Turdus merula )"

Article Title: Experimental Usutu virus infection in Eurasian blackbirds ( Turdus merula )

Journal: npj Viruses

doi: 10.1038/s44298-025-00133-w

Figure Legend Snippet: a Weight loss is expressed in % of initial weight. The humane endpoint was set at 75% of initial weight (dashed line). Results averaged from three individuals infected with USUV Afr3 and Eu3 and two control individuals, bars represent SD. Statistically significant difference was observed between the Eu3 and ctrls group on 6 d.p.i. as indicated by the asterisk * p = 0.03. b Survival curves of blackbirds. No statistically significant difference was observed in survival among the three groups of blackbirds.

Techniques Used: Infection, Control

GAMM fit on the individual blackbirds’ square-root transformed hourly-total moved distances (in meters) for each day. Due to the small sample size, we combined the Afr3 and Eu3 infected birds in one treatment group (USUV-infected), and compared these birds to the control group. For visualization purposes, the time of day is fixed to the average value within the total data set (whilst the model corrects for diurnal activity patterns). Intervals around the lines depict standard errors of the predicted daily values. The gray rectangle indicates the day on which the experimental infection took place.

Figure Legend Snippet: GAMM fit on the individual blackbirds’ square-root transformed hourly-total moved distances (in meters) for each day. Due to the small sample size, we combined the Afr3 and Eu3 infected birds in one treatment group (USUV-infected), and compared these birds to the control group. For visualization purposes, the time of day is fixed to the average value within the total data set (whilst the model corrects for diurnal activity patterns). Intervals around the lines depict standard errors of the predicted daily values. The gray rectangle indicates the day on which the experimental infection took place.

Techniques Used: Transformation Assay, Infection, Control, Activity Assay

Results averaged from three individuals infected with Afr3 and Eu3 viruses. a Levels of RNAemia expressed in Ct value. b Amount of virus shed through pharyngeal route expressed in Ct value, c Amount of virus shed through cloacal route expressed in Ct value. Error bars represent SD.

Figure Legend Snippet: Results averaged from three individuals infected with Afr3 and Eu3 viruses. a Levels of RNAemia expressed in Ct value. b Amount of virus shed through pharyngeal route expressed in Ct value, c Amount of virus shed through cloacal route expressed in Ct value. Error bars represent SD.

Techniques Used: Infection, Virus

a The amount of infectious virus in several tissues collected postmortem from the infected blackbirds is expressed in TCID 50 /g of tissue. Results averaged from three individuals for USUV Afr3 and USUV Eu3 groups, bars represent SD. A statistically significant difference (* p = 0.02) was observed between USUV Afr3 titer (2,3 × 10 9 TCID 50 /g) and USUV Eu3 titer (1 × 10 8 TCID 50 /g). b The amount of viral RNA detected in the lung and pallium of infected blackbirds is expressed in Ct value. Results averaged from three individuals for Afr3 and Eu3 groups, bars represent SD. No statistically significant differences were observed in either of the two tissues (lung p = 0.1; pallium p = 0.8).

Figure Legend Snippet: a The amount of infectious virus in several tissues collected postmortem from the infected blackbirds is expressed in TCID 50 /g of tissue. Results averaged from three individuals for USUV Afr3 and USUV Eu3 groups, bars represent SD. A statistically significant difference (* p = 0.02) was observed between USUV Afr3 titer (2,3 × 10 9 TCID 50 /g) and USUV Eu3 titer (1 × 10 8 TCID 50 /g). b The amount of viral RNA detected in the lung and pallium of infected blackbirds is expressed in Ct value. Results averaged from three individuals for Afr3 and Eu3 groups, bars represent SD. No statistically significant differences were observed in either of the two tissues (lung p = 0.1; pallium p = 0.8).

Techniques Used: Virus, Infection

USUV-associated lesions (H&E) ( a , c , e , g ) and USUV tropism ( b , d , f , h ) in the main target organs of USUV Afr3-infected blackbirds. a , b Spleen of blackbird n.4: a small multifocal areas of necrosis in the spleen (arrows) and infiltration of plasma cells and mott cells (arrowheads). b USUV antigen in the cytoplasm of large mononucleated cells morphologically compatible with splenic macrophages ( c , d ) Liver of blackbird n.4: c diffuse vacuolization and pigment deposition in the cytoplasm of hepatocytes, multiple cells with pyknotic nuclei and hyper eosinophilic cytoplasm (single cell necrosis) (arrowheads). d USUV antigen in the cytoplasm of hepatocytes (arrowhead), endothelial lining of the sinusoids (arrow) and Kupffer’s cells (asterisks). e , f Brain, pallium of blackbird n.1: e multiple neurons undergoing acidophilic neuronal necrosis (arrowheads) and mild perivascular infiltration of mononucleated leukocytes (arrow). f USUV antigen in the cytoplasm of neurons (arrowheads). g , h Brain, cerebellum of blackbird n.1: g Multiple Purkinje cells in variable degree of degeneration from chromatolysis to acidophilic neuronal necrosis (arrowheads). h USUV antigen in the cytoplasm of Purkinje cells (arrowhead) and adjacent neurons of the molecular layer (arrow).

Figure Legend Snippet: USUV-associated lesions (H&E) ( a , c , e , g ) and USUV tropism ( b , d , f , h ) in the main target organs of USUV Afr3-infected blackbirds. a , b Spleen of blackbird n.4: a small multifocal areas of necrosis in the spleen (arrows) and infiltration of plasma cells and mott cells (arrowheads). b USUV antigen in the cytoplasm of large mononucleated cells morphologically compatible with splenic macrophages ( c , d ) Liver of blackbird n.4: c diffuse vacuolization and pigment deposition in the cytoplasm of hepatocytes, multiple cells with pyknotic nuclei and hyper eosinophilic cytoplasm (single cell necrosis) (arrowheads). d USUV antigen in the cytoplasm of hepatocytes (arrowhead), endothelial lining of the sinusoids (arrow) and Kupffer’s cells (asterisks). e , f Brain, pallium of blackbird n.1: e multiple neurons undergoing acidophilic neuronal necrosis (arrowheads) and mild perivascular infiltration of mononucleated leukocytes (arrow). f USUV antigen in the cytoplasm of neurons (arrowheads). g , h Brain, cerebellum of blackbird n.1: g Multiple Purkinje cells in variable degree of degeneration from chromatolysis to acidophilic neuronal necrosis (arrowheads). h USUV antigen in the cytoplasm of Purkinje cells (arrowhead) and adjacent neurons of the molecular layer (arrow).

Techniques Used: Infection, Clinical Proteomics

Eight Eurasian blackbirds were sampled on day -7 (blood, serum, pharynx and cloacal swabs and breast feathers); after sampling, the blackbirds were singularly housed in cages in isolators and left to acclimatize for 7 days and activity and health status were monitored. On day 0, blackbirds were either inoculated with USUV Afr3 ( n = 3), Eu3 ( n = 3), or sterile PBS ( n = 2). On 1, 3 and 5 days post inoculation (d.p.i.) blackbirds were sampled (blood, serum, pharynx and cloacal swabs and breast feathers). At the humane endpoint, blackbirds were euthanized, necropsied, and tissue samples were collected for histopathology, tissue titration and RT-qPCR. Created in BioRender, agreement licence number OO28BUTARJ.

Figure Legend Snippet: Eight Eurasian blackbirds were sampled on day -7 (blood, serum, pharynx and cloacal swabs and breast feathers); after sampling, the blackbirds were singularly housed in cages in isolators and left to acclimatize for 7 days and activity and health status were monitored. On day 0, blackbirds were either inoculated with USUV Afr3 ( n = 3), Eu3 ( n = 3), or sterile PBS ( n = 2). On 1, 3 and 5 days post inoculation (d.p.i.) blackbirds were sampled (blood, serum, pharynx and cloacal swabs and breast feathers). At the humane endpoint, blackbirds were euthanized, necropsied, and tissue samples were collected for histopathology, tissue titration and RT-qPCR. Created in BioRender, agreement licence number OO28BUTARJ.

Techniques Used: Sampling, Activity Assay, Sterility, Histopathology, Titration, Quantitative RT-PCR

Isolation:

Article Title: Experimental Usutu virus infection in Eurasian blackbirds (Turdus merula).
Article Snippet: .. USUV Afr3 (isolated in 2016 from Turdus merula, GenBank accession MH891847.1) and USUV Eu3 (isolated in 2017 from Turdus merula, GenBank accessionMN122189.1) were grown and passaged three times on Vero cells (African green monkey kidney epithelial cells, ATCC CCL-81). .. Cells were inoculated at a multiplicity of infection (MOI) of 0.01 and incubated at 37 °C for 5–6 days in Dulbecco’s modified Eagle’s medium (DMEM; Lonza) with 2% FBS (Sigma-Aldrich), 100 U/ml penicillin, 100 μg/ml streptomycin (Lonza), 1% sodium bicarbonate (Lonza) and 2 mM L-glutamine (Lonza).

Article Title: Experimental Usutu virus infection in Eurasian blackbirds ( Turdus merula )
Article Snippet: .. USUV Afr3 (isolated in 2016 from Turdus merula , GenBank accession MH891847.1 ) and USUV Eu3 (isolated in 2017 from Turdus merula , GenBank accession MN122189.1 ) were grown and passaged three times on Vero cells (African green monkey kidney epithelial cells, ATCC CCL-81). .. Cells were inoculated at a multiplicity of infection (MOI) of 0.01 and incubated at 37 °C for 5–6 days in Dulbecco’s modified Eagle’s medium (DMEM; Lonza) with 2% FBS (Sigma-Aldrich), 100 U/ml penicillin, 100 μg/ml streptomycin (Lonza), 1% sodium bicarbonate (Lonza) and 2 mM L -glutamine (Lonza).

Image Search Results

Journal: npj Viruses

Article Title: Experimental Usutu virus infection in Eurasian blackbirds ( Turdus merula )

doi: 10.1038/s44298-025-00133-w

Figure Lengend Snippet: a Weight loss is expressed in % of initial weight. The humane endpoint was set at 75% of initial weight (dashed line). Results averaged from three individuals infected with USUV Afr3 and Eu3 and two control individuals, bars represent SD. Statistically significant difference was observed between the Eu3 and ctrls group on 6 d.p.i. as indicated by the asterisk * p = 0.03. b Survival curves of blackbirds. No statistically significant difference was observed in survival among the three groups of blackbirds.

Article Snippet: USUV Afr3 (isolated in 2016 from Turdus merula , GenBank accession MH891847.1 ) and USUV Eu3 (isolated in 2017 from Turdus merula , GenBank accession MN122189.1 ) were grown and passaged three times on Vero cells (African green monkey kidney epithelial cells, ATCC CCL-81).

Techniques: Infection, Control

Journal: npj Viruses

Article Title: Experimental Usutu virus infection in Eurasian blackbirds ( Turdus merula )

doi: 10.1038/s44298-025-00133-w

Figure Lengend Snippet: GAMM fit on the individual blackbirds’ square-root transformed hourly-total moved distances (in meters) for each day. Due to the small sample size, we combined the Afr3 and Eu3 infected birds in one treatment group (USUV-infected), and compared these birds to the control group. For visualization purposes, the time of day is fixed to the average value within the total data set (whilst the model corrects for diurnal activity patterns). Intervals around the lines depict standard errors of the predicted daily values. The gray rectangle indicates the day on which the experimental infection took place.

Techniques: Transformation Assay, Infection, Control, Activity Assay

Journal: npj Viruses

Article Title: Experimental Usutu virus infection in Eurasian blackbirds ( Turdus merula )

doi: 10.1038/s44298-025-00133-w

Figure Lengend Snippet: Results averaged from three individuals infected with Afr3 and Eu3 viruses. a Levels of RNAemia expressed in Ct value. b Amount of virus shed through pharyngeal route expressed in Ct value, c Amount of virus shed through cloacal route expressed in Ct value. Error bars represent SD.

Techniques: Infection, Virus

Journal: npj Viruses

Article Title: Experimental Usutu virus infection in Eurasian blackbirds ( Turdus merula )

doi: 10.1038/s44298-025-00133-w

Figure Lengend Snippet: a The amount of infectious virus in several tissues collected postmortem from the infected blackbirds is expressed in TCID 50 /g of tissue. Results averaged from three individuals for USUV Afr3 and USUV Eu3 groups, bars represent SD. A statistically significant difference (* p = 0.02) was observed between USUV Afr3 titer (2,3 × 10 9 TCID 50 /g) and USUV Eu3 titer (1 × 10 8 TCID 50 /g). b The amount of viral RNA detected in the lung and pallium of infected blackbirds is expressed in Ct value. Results averaged from three individuals for Afr3 and Eu3 groups, bars represent SD. No statistically significant differences were observed in either of the two tissues (lung p = 0.1; pallium p = 0.8).

Techniques: Virus, Infection

Journal: npj Viruses

Article Title: Experimental Usutu virus infection in Eurasian blackbirds ( Turdus merula )

doi: 10.1038/s44298-025-00133-w

Figure Lengend Snippet: USUV-associated lesions (H&E) ( a , c , e , g ) and USUV tropism ( b , d , f , h ) in the main target organs of USUV Afr3-infected blackbirds. a , b Spleen of blackbird n.4: a small multifocal areas of necrosis in the spleen (arrows) and infiltration of plasma cells and mott cells (arrowheads). b USUV antigen in the cytoplasm of large mononucleated cells morphologically compatible with splenic macrophages ( c , d ) Liver of blackbird n.4: c diffuse vacuolization and pigment deposition in the cytoplasm of hepatocytes, multiple cells with pyknotic nuclei and hyper eosinophilic cytoplasm (single cell necrosis) (arrowheads). d USUV antigen in the cytoplasm of hepatocytes (arrowhead), endothelial lining of the sinusoids (arrow) and Kupffer’s cells (asterisks). e , f Brain, pallium of blackbird n.1: e multiple neurons undergoing acidophilic neuronal necrosis (arrowheads) and mild perivascular infiltration of mononucleated leukocytes (arrow). f USUV antigen in the cytoplasm of neurons (arrowheads). g , h Brain, cerebellum of blackbird n.1: g Multiple Purkinje cells in variable degree of degeneration from chromatolysis to acidophilic neuronal necrosis (arrowheads). h USUV antigen in the cytoplasm of Purkinje cells (arrowhead) and adjacent neurons of the molecular layer (arrow).

Techniques: Infection, Clinical Proteomics

Journal: npj Viruses

Article Title: Experimental Usutu virus infection in Eurasian blackbirds ( Turdus merula )

doi: 10.1038/s44298-025-00133-w

Figure Lengend Snippet: Eight Eurasian blackbirds were sampled on day -7 (blood, serum, pharynx and cloacal swabs and breast feathers); after sampling, the blackbirds were singularly housed in cages in isolators and left to acclimatize for 7 days and activity and health status were monitored. On day 0, blackbirds were either inoculated with USUV Afr3 ( n = 3), Eu3 ( n = 3), or sterile PBS ( n = 2). On 1, 3 and 5 days post inoculation (d.p.i.) blackbirds were sampled (blood, serum, pharynx and cloacal swabs and breast feathers). At the humane endpoint, blackbirds were euthanized, necropsied, and tissue samples were collected for histopathology, tissue titration and RT-qPCR. Created in BioRender, agreement licence number OO28BUTARJ.

Techniques: Sampling, Activity Assay, Sterility, Histopathology, Titration, Quantitative RT-PCR

Review

usuv eu3 (ATCC)

Structured Review

Images

1) Product Images from "Experimental Usutu virus infection in Eurasian blackbirds ( Turdus merula )"

Related Articles

Isolation:

Similar Products

Image Search Results